Analysis Note

ControlPOSITIVE CONTROL:

Hela cells, neuronal tissues, antigen presenting dendritic cells.

Application

This Anti-Fascin Antibody, clone 55K2 is validated for use in WB, IC, IH(P) for the detection of Fascin.

Research Sub CategoryCytoskeleton

Research CategoryCell Structure

Western blot. Recognizes a protein of 55 kDa. Suggested working dilution is 1:100-1:1,000. Suggested blocking buffer is 3% BSA in PBS or 3% non fat milk in PBS.

Immunohistochemistry on formalin fixed, paraffin embedded tissue sections. Suggested working dilution is 1:100-1:1,000.

Immunocytochemistry on cold methanol fixed cultured cells. Suggested working dilution is 1:100-1:1,000.

Optimal working dilutions must be determined by the end user.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Human fascin

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Liquid in 0.02M Phosphate buffer with 0.25M NaCl and 0.1% sodium azide.

Format: Purified

Specificity

Fascin. The antibody is strongly positive when antigen-presenting dendritic cells are matured. MAB3582 is a sensitive marker for Reed-Sternberg Cells of Hodgkin′s Disease. The protein is an actin bundling protein specifically localized in filopodia and growth cones.

Storage and Stability

Maintain at 2-8°C in undiluted aliquots up to 6 months after date of receipt.